Caldesmon 1 (CALD1) antibody

Antigen: Caldesmon 1 (CALD1)

Synonyms: CDM, H-CAD, L-CAD, NAG22, MGC21352, AI195384, AV071549, AW536160, MGC30319, 4833423D12Rik, C920027I18Rik, CALD1, caldesmon, zgc:56389, si:bz30i22.4, si:rp71-30i22.4, DKFZp459K021

Clonality: Monoclonal (H-CALD)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN95903

Additional Information

Alternative name: Caldesmon

Immunogen: BALB/C mice were injected with crude human uterus extract.

Format: Ig-fraction

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: H-CALD

Specificity: This antibody is specific to 150 kD protein known as h-caldesmon. Caldesmon is involved in smooth muscle and non-muscle contraction. Two closely related variants of human Caldesmon have been identified. The h-caldesmon variant (120-150 kD) is predominantly expressed in smooth muscle, whereas l-caldesmon (70-80 kD) is found in non-muscle tissue and cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Uterus. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Frid, Shekhonin, Koteliansky et al.: "Phenotypic changes of human smooth muscle cells during development: late expression of heavy caldesmon and calponin." in: Developmental biology, Vol. 153, Issue 2, pp. 185-93, 1992 (PubMed).

Sobue, Sellers: "Caldesmon, a novel regulatory protein in smooth muscle and nonmuscle actomyosin systems." in: The Journal of biological chemistry, Vol. 266, Issue 19, pp. 12115-8, 1991 (PubMed).