Carcinoembryonic Antigen (CEA) antibody

Antigen: Carcinoembryonic Antigen (CEA)

Synonyms: CEACAM5, CEA, CEACAM, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik

Clonality: Monoclonal (Col-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96052

Additional Information

Immunogen: BALB/C mice were injected with extract of colon carcinoma cells.

Format: Supernatant (prediluted)

Isotype: IgG2a kappa

Clone: Col-1

Specificity: This antibody reacts with a highly glycosylated protein of MW 180 kD, synthesized during development in the fetal gut and several tumors. It does not react with normal colon antigen (NCA). It stains the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Colan carcinoma. Cellular localization: Cytoplasmic, lumenal membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Pfaltz, Odermatt, Christen et al.: "Immunohistochemistry in the diagnosis of malignant mesothelioma." in: Virchows Archiv. A, Pathological anatomy and histopathology, Vol. 411, Issue 4, pp. 387-93, 1987 (PubMed).

Ellis, Hitchcock: "Tumour marker immunoreactivity in adenocarcinoma." in: Journal of clinical pathology, Vol. 41, Issue 10, pp. 1064-7, 1989 (PubMed).

Albers, Fleuren, Escribano et al.: "Immunohistochemistry of CEA in the human pancreas during development, in the adult, chronic pancreatitis, and pancreatic adenocarcinoma." in: American journal of clinical pathology, Vol. 90, Issue 1, pp. 17-22, 1988 (PubMed).

Oikawa, Imajo, Noguchi et al.: "The carcinoembryonic antigen (CEA) contains multiple immunoglobulin-like domains." in: Biochemical and biophysical research communications, Vol. 144, Issue 2, pp. 634-42, 1987 (PubMed).