Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1) antibody

Antigen: Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1)

Synonyms: ICE, P45, IL1BC, Il1bc, Ice, DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370, CASP1, LOC100033888

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN96090

Additional Information

Alternative name: Caspase 1/ Interleukin-1beta Converting Enzyme

Immunogen: A synthetic peptide from the second quarter of human Caspase 1 protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 45 kD protein, Caspase 1 and is expressed as a proenzyme in many tissues. Active Caspase 1 is a tetramer of two subunits p10 and p20 in 2:2 ratio. Overexpression of Caspase 1 can induce apoptosis in fibroblast, which can be inhibited by Overexpression of Crm A, a protein from pox virus. This antibody cross reacts with human, mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Placenta. Cellular localization: Nuclear, cell membrane Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Caspase 1 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human placenta stained with Caspase 1 antibody

Publications

Publications: Enari, Hug, Nagata: "Involvement of an ICE-like protease in Fas-mediated apoptosis." in: Nature, Vol. 375, Issue 6526, pp. 78-81, 1995 (PubMed).

Walker, Talanian, Brady et al.: "Crystal structure of the cysteine protease interleukin-1 beta-converting enzyme: a (p20/p10)2 homodimer." in: Cell, Vol. 78, Issue 2, pp. 343-52, 1994 (PubMed).