Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) antibody

Antigen: Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)

Synonyms: MCH2, Mch2, mCASP-6, MGC93335, xCaspase-6, mch2, caspase-6, CASP6, MGC127633, zgc:112960, casp6

Clonality: Monoclonal (6CSP03)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96097

Additional Information

Alternative name: Caspase 6

Immunogen: BALB/C mice were immunized with recombinant human Caspase 6 prodomain.

Format: Purified

Isotype: IgG1 kappa

Clone: 6CSP03

Specificity: This antibody stains with a 34 kD protein, known as Caspase 6. Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells and are activated in response to distinct apoptosis-inducing stimuli. Caspase 6 cleaves nuclear mitotic apparatus protein and medicates the shrinkage and fragmentation of nuclei.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Purification: Purified

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Fernandes-Alnemri, Litwack, Alnemri: "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family." in: Cancer research, Vol. 55, Issue 13, pp. 2737-42, 1995 (PubMed).