Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody

Antigen: Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9)

Synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c, Mch6, AI115399, AW493809, Caspase-9, CASP9, LOC100101592

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96117

Additional Information

Alternative name: Caspase 9

Immunogen: A synthetic peptide from the middle of human Caspase 9.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 46 - 48 kD protein. Activation of procaspase 9 by Apaf-1 requires proteolytic cleavage to generate the mature Caspase molecule. Once activated, Caspase 9 can initiate a Caspase cascade involving the downstream executioners Caspase 3, 6 and 7. Caspase 9 is essential for apoptosis during the normal development of the central nervous system.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Stomach. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Purification: Ig-fraction,Purified

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Caspase 9 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Pan, ORourke, Dixit: "Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex." in: The Journal of biological chemistry, Vol. 273, Issue 10, pp. 5841-5, 1998 (PubMed).

Kuida, Haydar, Kuan et al.: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9." in: Cell, Vol. 94, Issue 3, pp. 325-37, 1998 (PubMed).

Hakem, Hakem, Duncan et al.: "Differential requirement for caspase 9 in apoptotic pathways in vivo." in: Cell, Vol. 94, Issue 3, pp. 339-52, 1998 (PubMed).