CD1a Molecule (CD1A) antibody

Antigen: CD1a Molecule (CD1A)

Synonyms: R4, T6, CD1, FCB6, HTA1, CD1A, CD1.1, CD1.2, CD1A2

Clonality: Monoclonal (1CA04)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96184

Additional Information

Alternative name: CD1a

Immunogen: BALB/C mice were injected with thymus cells.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 1CA04

Specificity: This antibody reacts with a 49 kD protein. At least five CD1 genes are identified (CD1a, b, c, d and e) . This antibody reacts with cortical thymocytes, Langerhans cells in epidermis, dendritic cells of dermis and Langerhans cells of mucosa of tonsil. This antibody may be useful in the characterization of leukemias and lymphomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane, cytoplasm

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Bobryshev, Lord, Rainer et al.: "Vascular dendritic cells and atherosclerosis." in: Pathology, research and practice, Vol. 192, Issue 5, pp. 462-7, 1997 (PubMed).

Mazal, Hainfellner, Preiser et al.: "Langerhans cell histiocytosis of the hypothalamus: diagnostic value of immunohistochemistry." in: Clinical neuropathology, Vol. 15, Issue 2, pp. 87-91, 1996 (PubMed).

Aractingi, Gluckman, Dauge-Geffroy et al.: "Langerhans' cells are depleted in chronic graft versus host disease." in: Journal of clinical pathology, Vol. 50, Issue 4, pp. 305-9, 1997 (PubMed).