CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96233

Additional Information

Alternative name: CD3

Immunogen: Synthetic human CD3 polypeptide conjugated to bovine serum albumin.

Format: Ig-fraction, Liquid (prediluted)

Specificity: This antibody reacts with intracytoplasmic portion of the CD3 antigen on the T cells. It stains T cells in cortex as well as medulla of the thymus and lymphoid tissues. This antibody also labels T cell neoplasm, malignant histiocytes and in Hodgkin's disease.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Mason, Krissansen, Davey et al.: "Antisera against epitopes resistant to denaturation on T3 (CD3) antigen can detect reactive and neoplastic T cells in paraffin embedded tissue biopsy specimens." in: Journal of clinical pathology, Vol. 41, Issue 2, pp. 121-7, 1988 (PubMed).

Mason, Cordell, Brown et al.: "Detection of T cells in paraffin wax embedded tissue using antibodies against a peptide sequence from the CD3 antigen." in: Journal of clinical pathology, Vol. 42, Issue 11, pp. 1194-200, 1990 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).