CD247 Molecule (CD247) antibody

Antigen: CD247 Molecule (CD247)

Synonyms: T3Z, CD3H, CD3Q, CD3Z, TCRZ, CD3-ZETA, Cd3, T3z, Cd3h, Cd3z, TCRk, Tcrz, CD3-eta, CD3zeta, AW552088, CD3-zeta, 4930549J05Rik, A430104F18Rik, CD247, im:7156386, LOC100136514, LOC100136549, LOC100305023

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96237

Additional Information

Alternative name: CD3 zeta

Immunogen: A synthetic peptide corresponding to aa 36 54 aa of the human CD3 zeta protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 19 kD protein. CD3 consists of five invariant polypeptide chains that associate to form three dimmers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. Zeta chain consists of three consecutive copies of the same motif. This antibody cross reacts with mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against CD3 zeta containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union