CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody

Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)

Synonyms: T3E, TCRE, FLJ18683, CD3E, cd3e, CD3, t3e, tcre

Clonality: Monoclonal (500A2)

Host: Syrian Hamster

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN96240

Additional Information

Alternative name: CD3Epsilon

Immunogen: Mouse T-cell receptor Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 500A2

Specificity: Syrian Hamster Anti-Mouse CD3e (Clone 500 A2) recognizes an epitope on Mouse CD3e. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution: The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Application Details

Application Notes: IHC (Frozen), FC, IP, FA Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a working dilution of 1:10 - 1:50. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3e. A suggested method would be to stain cells expressing CD3e with =0.5 g per 1 x 106 cells in a total staining volume of 100 l followed by staining with a suitable secondary antibody. Functional Assay: Binding of Syrian Hamster Anti-Mouse CD3e (Clone 500A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation of mouse T cells. However, it is recommended to use Anti-Mouse CD3e Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219) for functional assays. Each Investigator should determine their own optimal working dilution for specific applications.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Stability This antibody is stable when stored undiluted at 2-8C. For long term storage, aliquo

Storage: This antibody is stable when stored undiluted at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Research Area: CD Antigens

Restrictions: For Research Use only