CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (1F6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96242

Additional Information

Alternative name: CD4

Immunogen: Synthetic peptide corresponding to the external domain of CD4 molecule.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1F6

Specificity: This antibody reacts specifically with CD4 cells. CD4 is a single chain transmembrane glycoprotein with a molecular weight of 59 kD. The CD4 antigen is found on a T cell subset (helper/inducer) and also on thymocytes and monocytes.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking technique is required for paraffin embedded tissue sections. We recommend using 1mM EDTA pH 8.0 buffer instead of sodium citrate pH 6.0 buffer for antigen unmasking. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Gay, Maddon, Sekaly et al.: "Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen." in: Nature, Vol. 328, Issue 6131, pp. 626-9, 1987 (PubMed).

Dalgleish, Beverley, Clapham et al.: "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus." in: Nature, Vol. 312, Issue 5996, pp. 763-7, 1985 (PubMed).

Stein, Lennert, Feller et al.: "Immunohistological analysis of human lymphoma: correlation of histological and immunological categories." in: Advances in cancer research, Vol. 42, pp. 67-147, 1985 (PubMed).