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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (1F6)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
3 references available
Catalog no. ABIN96243
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 389.40 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CD4
Immunogen Synthetic peptide corresponding to the external domain of CD4 molecule.
Format Ig-fraction (prediluted)
Isotype IgG1
Clone 1F6
Specificity This antibody reacts specifically with CD4 cells. CD4 is a single chain transmembrane glycoprotein with a molecular weight of 59 kD. The CD4 antigen is found on a T cell subset (helper/inducer) and also on thymocytes and monocytes.

Application Details
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Application Notes Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking technique is required for paraffin embedded tissue sections. We recommend using 1mM EDTA pH 8.0 buffer instead of sodium citrate pH 6.0 buffer for antigen unmasking. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 1 to 4 ug/ml
Purification Ig-fraction (prediluted)
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Variants back to top

Gay, Maddon, Sekaly et al.: "Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen." in: Nature, Vol. 328, Issue 6131, pp. 626-9, 1987 (PubMed).

Dalgleish, Beverley, Clapham et al.: "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus." in: Nature, Vol. 312, Issue 5996, pp. 763-7, 1985 (PubMed).

Stein, Lennert, Feller et al.: "Immunohistological analysis of human lymphoma: correlation of histological and immunological categories." in: Advances in cancer research, Vol. 42, pp. 67-147, 1985 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (497), Rat (208), Rabbit (105), Goat (2)
Reactivities Human (397), Mouse (Murine) (244), Rat (Rattus) (116), Cow (Bovine) (36), Chicken (35), Dog (Canine) (35), Guinea Pig (26), Sheep (Ovine) (25), Pig (Porcine) (24), Cat (Feline) (23), Bird (Avian) (8), Rabbit (5), Duck (3), Horse (Equine) (3), Rhesus Monkey (3), Monkey (2), Goat (1)
Applications Flow Cytometry (FACS) (635), Western Blotting (WB) (118), Immunoprecipitation (IP) (96), Immunohistochemistry (IHC) (88), ELISA (85), Immunofluorescence (IF) (73), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunohistochemistry (Fixed) (IHC (fx)) (71), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Immunocytochemistry (ICC) (39), Functional Studies (Func) (36), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (11), Cytotoxicity Test (CyTox) (10), Blocking Antibody (Inhibition) (5), Blocking Antibody (Inhibition) (4), Immunostaining (ISt) (4), Activation (Act) (3), Immunoelectron Microscopy (IEM) (3), Neutralization (Neut) (2)
Conjugates FITC (126), PE (85), Biotin (82), APC (48), RPE (22), Cy5 (10), Cy5,PE (10), PerCP (10), Cy5.5 (7), Cy7,PE (6), HRP (5), PE,Cy7 (5), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy7 (3), Gold (3), PE,Cy5 (3), PE-Dyomics 647 (3), PerCP,Cy5.5 (3), SPRD (3), Alexa Fluor 700 (2), PE,Cy3 (2), PE,DY647 (2), PE-Cy5 (2), PE-Dyomics 590 (2), PE-Dyomics 747 (2), Pacific Blue (2), Allophycocyanin (1), CF405M (1), Cy5.5,PerCP (1), DY647 (1), Dyomics 647 (1), PE,Cy5.18 (CyQ) (1), PE,DY590 (1), PE,DyLight 594 (1), PE,Dyomics 647 (1), PE,Texas Red (TR) (1), PE-Cy3 (1), PE-Cy5.5 (1), PE-Cy7 (1), PE-DyLight 594 (1), PerCP-Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1), Un-conjugated (1)
Epitopes N-Term (26), Domain 1 (10), Transcript Variant 1 (9), C-Term (7), Domain 2 (5), Non-Polymorphic Site (3), AA 26-226 (2), AA 414-433 (2), Domain 3,Domain 4 (2), AA 325-375 (1), Cytoplasmic Domain (1), Extracellular Domain (1), Internal Region (1), pSer433 (1)