Angiopoietin 1 antibody (AA 76-85)

Catalog No. ABIN962636

Product Details anti-Angiopoietin 1 Antibody

Target: Angiopoietin 1 (ANGPT1)

Binding Specificity: AA 76-85

Reactivity: Human, Mouse, Dog, Rabbit, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ANGPT1 / Angiopoietin-1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit, Opossum (100%) Rat, Sheep, Bovine, Pig, Xenopus (90%) Arabidopsis, Paramecium (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PDFSSQKLQH from an internal region of human ANGPT1 / Angiopoietin-1 (NP_001137.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit, Opossum (100%), Rat, Sheep, Bovine, Pig, Xenopus (90%), Arabidopsis, Paramecium (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48kD was observed. No bands were observed in mock-transfected HEK293 and the 48kD plus a 75kD band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1 / ANG1 / Angiopoietin-1 (ANGPT1 Products)

Synonyms: AGP1 antibody, AGPT antibody, ANG1 antibody, 1110046O21Rik antibody, Ang-1 antibody, Ang1 antibody, Agpt antibody, Agpt1 antibody, ang1 antibody, wu:fc76g06 antibody, ANG-1 antibody, angpt1 antibody, MGC80788 antibody, angiopoietin 1 antibody, angiopoietin 1 S homeolog antibody, ANGPT1 antibody, Angpt1 antibody, angpt1 antibody, angpt1.S antibody

Background: Name/Gene ID: ANGPT1

Synonyms: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003

Gene ID: 284

NCBI Accession: NP_001137

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process