ATP1B1 antibody (AA 85-99)

Catalog No. ABIN962639

Product Details anti-ATP1B1 Antibody

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Binding Specificity: AA 85-99

Reactivity: Human, Mouse, Rat, Dog, Horse, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ATP1B1. This antibody is expected to recognize both reported isoforms (NP_001668.1, NP_001001787.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Horse (100%) Marmoset, Elephant, Panda, Pig, Guinea pig (93%) Sheep, Bovine, Rabbit (87%) Chicken (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KTEISFRPNDPKSYE from an internal region of human ATP1B1 (NP_001668.1, NP_001001787.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Horse (100%), Marmoset, Elephant, Panda, Pig, Guinea pig (93%), Sheep, Bovine, Rabbit (87%), Chicken (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48  kDa band observed in Rat Retina lysates and 50  kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1  kDa according to Human NP_001668.1, 35.2  kDa according to Mouse NP_033851.1 and Rat NP_037245.2).The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: ATP1B1 (ATP1B1 Products)

Synonyms: ATP1B antibody, Atp4b antibody, Atpb antibody, Atpb-1 antibody, NKbeta1 antibody, ATPBS antibody, atp1b1 antibody, atp1b1a antibody, cb710 antibody, ATPase Na+/K+ transporting subunit beta 1 antibody, ATPase, Na+/K+ transporting, beta 1 polypeptide antibody, ATPase beta chain antibody, ATP synthase CF1 beta subunit antibody, ATPase Na+/K+ transporting subunit beta 1 S homeolog antibody, ATPase, Na+/K+ transporting, beta 1b polypeptide antibody, ATP1B1 antibody, Atp1b1 antibody, atpB antibody, atp1b1.S antibody, atp1b1b antibody

Background: Name/Gene ID: ATP1B1
Subfamily: ATPase - P type, beta subunit
Family: Transporter

Synonyms: ATP1B1, Adenosinetriphosphatase, ATP1B, Atpb1

Gene ID: 481

NCBI Accession: NP_001668, NP_001001787

UniProt: P05026

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome