CHRNB4 antibody (AA 87-101)

Catalog No. ABIN962642

Product Details anti-CHRNB4 Antibody

Target: CHRNB4 (Cholinergic Receptor, Nicotinic, beta 4 (Neuronal) (CHRNB4))

Binding Specificity: AA 87-101

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNB4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CHRNB4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Elephant, Panda, Dog, Bovine, Pig (93%) Mouse, Rat, Hamster, Horse, Opossum (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DYRLTWNSSRYEGVN from an internal region of human CHRNB4 (NP_000741.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Elephant, Panda, Dog, Bovine, Pig (93%), Mouse, Rat, Hamster, Horse, Opossum (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 40kD band in human testis lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kD according to NP_000741.1. The 40kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHRNB4

Target: CHRNB4 (Cholinergic Receptor, Nicotinic, beta 4 (Neuronal) (CHRNB4))

Alternative Name: CHRNB4 (CHRNB4 Products)

Synonyms: Acrb-4 antibody, Acrb4 antibody, cholinergic receptor nicotinic beta 4 subunit antibody, cholinergic receptor, nicotinic, beta polypeptide 4 antibody, CHRNB4 antibody, Chrnb4 antibody

Background: Name/Gene ID: CHRNB4
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNB4, AChR-beta4, NAChR beta 4

Gene ID: 1143

NCBI Accession: NP_000741

UniProt: P30926