DAP3 antibody (AA 387-398)

Catalog No. ABIN962650

Product Details anti-DAP3 Antibody

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Binding Specificity: AA 387-398

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This DAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DAP3. This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Bovine (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide NPSLLERHCAYL from the C-terminus of human DAP3 (NP_387506.1, NP_001186779.1, NP_001186780.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Bovine (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DAP3

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Alternative Name: DAP3 (DAP3 Products)

Synonyms: DAP-3 antibody, MRP-S29 antibody, MRPS29 antibody, bMRP-10 antibody, 4921514D13Rik antibody, im:6905684 antibody, death associated protein 3 antibody, DAP3 antibody, Dap3 antibody, dap3 antibody

Background: Name/Gene ID: DAP3

Synonyms: DAP3, Death associated protein 3, Death-associated protein 3, MRP-S29, BMRP-10, DAP-3, MRPS29, S29mt

Gene ID: 7818

NCBI Accession: NP_387506, NP_001186779, NP_001186780

UniProt: P51398