MSH5 antibody (AA 508-519)

Catalog No. ABIN962670

Product Details anti-MSH5 Antibody

Target: MSH5 (MutS Homolog 5 (MSH5))

Binding Specificity: AA 508-519

Reactivity: Human, Mouse, Chicken, Monkey, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This MSH5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human MSH5. This antibody is expected to recognise three reported isoforms ( NP_079535.3 , NP_751897.1, NP_002432.1), Reported variants NP_751898.1 and NP_002432.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%) Rat, Panda, Bovine, Dog, Horse, Pig (92%) Marmoset (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 , NP_751897.1, NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%), Rat, Panda, Bovine, Dog, Horse, Pig (92%), Marmoset (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:64000)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MSH5

Target: MSH5 (MutS Homolog 5 (MSH5))

Alternative Name: MSH5 (MSH5 Products)

Synonyms: MSH5 antibody, G7 antibody, MUTSH5 antibody, NG23 antibody, Mut5 antibody, mutS homolog 5 antibody, mutS protein homolog 5 antibody, MSH (MutS Homolog) family antibody, MSH5 antibody, msh5 antibody, LOC100635155 antibody, Msh5 antibody, msh-5 antibody

Background: Name/Gene ID: MSH5

Synonyms: MSH5, MutS homolog 5 (E. coli), HMSH5, MutS protein homolog 5, NG23, G7, MutS (E. coli) homolog 5, MUTSH5

Gene ID: 4439

NCBI Accession: NP_079535, NP_751897, NP_002432

Pathways: M Phase