CD5 Molecule (CD5) antibody
| Antigen |
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| Synonyms |
T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (182), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (295), Immunohistochemistry (Frozen Sections) (IHC (fro)) (78), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN96279 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
508.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD5
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Immunogen
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A synthetic peptide from the intracellular region of CD5.
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Format
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Ig-fraction, Purified
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Specificity
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CD5 is a transmembrane protein and is expressed on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against CD5 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD5 Molecule (CD5)", type "Antibodies"
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Hosts
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Mouse (265), Rat (55), Rabbit (48)
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Reactivities
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Human (182), Mouse (Murine) (82), Rat (Rattus) (48), Cow (Bovine) (26), Cat (Feline) (24), Sheep (Ovine) (24), Chicken (20), Horse (Equine) (19), Rabbit (15), Dog (Canine) (12), Pig (Porcine) (7), Bird (Avian) (4), Goat (1), Monkey (1)
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Applications
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Flow Cytometry (FACS) (295), Immunohistochemistry (Frozen Sections) (IHC (fro)) (78), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (53), Immunofluorescence (IF) (35), Functional Studies (Func) (15), Immunohistochemistry (IHC) (13), Cytotoxicity Test (CyTox) (8), Immunocytochemistry (ICC) (5), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (65), Biotin (43), PE (42), APC (15), RPE (10), Cy5.5 (3), Cy7,PE (3), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1), SPRD (1)
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