V5 Epitope Tag antibody (Biotin)

Details for Product No. ABIN962841, Supplier: Log in to see
Antigen
Reactivity
Tag
403
18
16
8
3
3
Host
Mouse
248
144
37
7
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
31
28
20
14
13
12
12
12
11
10
10
10
10
9
8
8
7
6
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
369
263
137
95
78
71
53
44
28
13
9
8
5
4
4
2
2
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Brand THE™
Immunogen V5 tag peptide conjugated - KLH
Sequence GKPIPNPLLG LDST
Clone 4C12E11
Isotype IgG2a
Specificity THETM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
Characteristics The THETM V5 Tag Antibody [Biotin], mAb, Mouse is THETM V5 Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Purification Protein A affinity column
Alternative Name V5 Tag
Target Type Tag
Background V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.005-0.05 µg/mL
Western blot: 0.2-1 µg/mL
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Buffer PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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