V5 Epitope Tag antibody (HRP)

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Antigen
Reactivity
Tag
404
3
3
3
3
Host
Mouse
220
135
37
9
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
28
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
342
265
123
92
71
65
53
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Brand THE™
Immunogen V5 tag peptide conjugated - KLH
Sequence GKPIPNPLLG LDST
Clone 4C12E11
Isotype IgG2a
Specificity THETM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
Characteristics The THETM V5 Tag Antibody [HRP], mAb, Mouse is THETM V5 Tag Antibody, mAb, Mouse conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Purification Protein A affinity column
Alternative Name V5 Tag
Target Type Tag
Background V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL.Western blot: 0.2-1 µg/mL.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Buffer PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.