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V5 Epitope Tag antibody (HRP)

WB, ELISA Host: Mouse Monoclonal 4C12E11 HRP
Catalog No. ABIN962844
  • Target See all V5 Epitope Tag products
    V5 Epitope Tag
    Reactivity
    Please inquire
    Host
    • 60
    • 40
    • 10
    • 7
    • 6
    • 2
    • 1
    Mouse
    Clonality
    • 57
    • 56
    • 10
    Monoclonal
    Conjugate
    • 66
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This V5 Epitope Tag antibody is conjugated to HRP
    Application
    • 100
    • 58
    • 44
    • 44
    • 31
    • 30
    • 13
    • 10
    • 8
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Brand
    THE™
    Sequence
    GKPIPNPLLG LDST
    Specificity
    THETM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
    Characteristics
    The THETM V5 Tag Antibody [HRP], mAb, Mouse is THETM V5 Tag Antibody, mAb, Mouse conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
    Purification
    Protein A affinity column
    Immunogen
    V5 tag peptide conjugated - KLH
    Clone
    4C12E11
    Isotype
    IgG2a
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.1 µg/mL.Western blot: 0.2-1 µg/mL.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Target
    V5 Epitope Tag
    Alternative Name
    V5 Tag (V5 Epitope Tag Products)
    Target Type
    Tag
    Background
    V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
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