Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD8a Molecule (CD8A) antibody

Antigen

CD8a Molecule (CD8A)
Synonyms CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Clonality Monoclonal (144B)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1 reference available
Catalog no. ABIN96305
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 272.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 5 Business Days

Additional Information
Application Details Publications Alternatives Secondary Antibodies Variants back to top

Alternative name CD8
Immunogen Synthetic peptide comprising of 13 C terminal amino acids of the cytoplasmic domain of CD8 molecule.
Format Supernatant (prediluted)
Isotype IgG1 kappa  (Matching secondary antibodies)
Clone 144B
Specificity This antibody reacts specifically with a 32 kD protein similar to CD8 molecule. This antibody stains cytotoxic/suppressor T cells and shows a staining patterns similar to clustered CD8 monoclonal antibodies.

Application Details
Publications Alternatives Secondary Antibodies Variants back to top

Application Notes Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area CD Antigens
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Secondary Antibodies Variants back to top

Publications Mason, Cordell, Gaulard et al.: "Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence." in: Journal of clinical pathology, Vol. 45, Issue 12, pp. 1084-8, 1993 (PubMed).

Alternatives
Application Details Publications Secondary Antibodies Variants back to top

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"
Hosts Mouse (310), Rat (106), Rabbit (82)
Reactivities Human (288), Mouse (Murine) (121), Rat (Rattus) (50), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Applications Flow Cytometry (FACS) (388), Immunohistochemistry (Frozen Sections) (IHC (fro)) (131), Immunoprecipitation (IP) (76), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (50), Western Blotting (WB) (43), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Enzyme Immunoassay (EIA) (5), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), To be determined by user (TBD) (1)
Conjugates FITC (84), Biotin (47), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), PerCP (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitopes C-Term (1), N-Term (1)