CD10 antibody

Antigen: CD10

Clonality: Monoclonal (56C6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96347

Additional Information

Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the CD10 glycoprotein.

Format: Supernatant (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 56C6

Specificity: This antibody is specific to human CD10 antigen of 100 kD, also known as common acute lymphocytic leukemia antigen (CALLA). CD10 antigen has been identified on the surface of early lymphoid progenitor cells, immature B cells with in adult bone marrow and germinal center B cells with in lymphoid tissue.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Kiyokawa, Kokai, Ishimoto et al.: "Characterization of the common acute lymphoblastic leukaemia antigen (CD10) as an activation molecule on mature human B cells." in: Clinical and experimental immunology, Vol. 79, Issue 3, pp. 322-7, 1990 (PubMed).

Haralambidou, Melo, Catovsky: "Different reactivity of monoclonal antibodies against common acute lymphoblastic leukaemia antigen (CD10)." in: Journal of clinical pathology, Vol. 40, Issue 5, pp. 490-3, 1987 (PubMed).

Carrel, Zografos, Schreyer et al.: "Expression of CALLA/CD10 on human melanoma cells." in: Melanoma research, Vol. 3, Issue 5, pp. 319-23, 1994 (PubMed).

Scheuermann, Racila: "CD19 antigen in leukemia and lymphoma diagnosis and immunotherapy." in: Leukemia & lymphoma, Vol. 18, Issue 5-6, pp. 385-97, 1996 (PubMed).