Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody

Antigen: Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4)

Synonyms: LeX, CD15, ELFT, FCT3A, FUTIV, SSEA-1, FUC-TIV

Clonality: Monoclonal (MY-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96433

Additional Information

Alternative name: CD15

Immunogen: Purified neutrophils from normal human peripheral blood.

Format: Supernatant (prediluted)

Isotype: IgM kappa  (Matching secondary antibodies)

Clone: MY-1

Specificity: The specificity of this antibody is similar to antibodies Leu-M1, T 9 and VIM-D5, recognizing an epitope involving the carbohydrate sequence 3-fucosyl-N-acetyllacetosamine. This antibody reacts with an antigen located on mature granulocytes and on Hodgkin and Reed-Sternberg cells.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Stein, Hansmann, Lennert et al.: "Reed-Sternberg and Hodgkin cells in lymphocyte-predominant Hodgkin's disease of nodular subtype contain J chain." in: American journal of clinical pathology, Vol. 86, Issue 3, pp. 292-7, 1986 (PubMed).

Dorfman, Gatter, Pulford et al.: "An evaluation of the utility of anti-granulocyte and anti-leukocyte monoclonal antibodies in the diagnosis of Hodgkin's disease." in: The American journal of pathology, Vol. 123, Issue 3, pp. 508-19, 1986 (PubMed).

Stein, Uchuanska-Ziegler, Gerdes et al.: "Hodgkin and Sternberg-Reed cells contain antigens specific to late cells of granulopoiesis." in: International journal of cancer. Journal international du cancer, Vol. 29, Issue 3, pp. 283-90, 1982 (PubMed).

Stein, Gerdes, Schwab et al.: "Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population." in: International journal of cancer. Journal international du cancer, Vol. 30, Issue 4, pp. 445-59, 1983 (PubMed).