AHSA1 antibody
-
- Target See all AHSA1 Antibodies
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
-
Reactivity
- Human, Mouse, Chimpanzee
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This AHSA1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human AHA1 protein. A BLAST analysis was used to suggest cross-reactivity with AHA1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Cross-Reactivity
- Human, Chimpanzee
- Characteristics
- Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
- Purification
- affinity purified
- Sterility
- Sterile filtered
- Immunogen
-
This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein - Clone
- 25F2-D9
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product AHSA1 Primary Antibody
-
-
- Application Notes
- This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.
- Comment
-
Gene Name: Ahsa1
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
-
- Target
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
- Alternative Name
- AHA1 (AHSA1 Products)
- Synonyms
- ahsa1 antibody, MGC52884 antibody, MGC132147 antibody, MGC80312 antibody, AHSA1 antibody, MGC89589 antibody, wu:fb83b05 antibody, zgc:136821 antibody, zgc:56075 antibody, AHA1 antibody, C14orf3 antibody, p38 antibody, BC023857 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 S homeolog antibody, activator of Hsp90 ATPase activity 1 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 L homeolog antibody, activator of HSP90 ATPase activity 1 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 antibody, AHA1, activator of heat shock protein ATPase homolog 1a antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) antibody, AHA1, activator of heat shock protein ATPase 1 antibody, ahsa1.S antibody, AHSA1 antibody, ahsa1.L antibody, ahsa1 antibody, ahsa1a antibody, Ahsa1 antibody
- Background
-
Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Synonyms: Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody - Gene ID
- 217737
- NCBI Accession
- NP_666148
- UniProt
- Q8BK64
-