IL-2 antibody

Catalog No. ABIN964739

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for cat IL-2 protein. A BLAST analysis was used to suggest cross-reactivity with IL-2 from cat, dog, wild boar, horse, rhesus monkey, baboon, and human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-2 from other sources has not been determined.

Characteristics: IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common gamma chain (gammac). IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and the common gamma chain (gammac). The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, gammadelta T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.

Purification: purified

Sterility: Sterile filtered

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: IL2

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Synonyms: IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Background: IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common gamma chain (γc). IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and the common gamma chain (γc). The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, γδ T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.
Synonyms: Interleukin-2, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

Gene ID: 751114

NCBI Accession: NP_001036802

UniProt: Q07885

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation