Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyms: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Clonality: Monoclonal (L26)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96485

Additional Information

Alternative name: CD20

Immunogen: BALB/C mice were injected with human tonsil B cells.

Format: Supernatant

Isotype: IgG2a kappa

Clone: L26

Specificity: This antibody is specific to a 33 kD polypeptide present on the majority of B cells in peripheral blood and lymphoid tissue and also with a minor component of 30 kD. No reactivity with other hematopoietic cells has been observed.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Cell membrane and cytoplasm Other Applications: Immunoprecipitation and western blotting (1:100-750).

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CD20, B cell antibody (ABIN96485)

Western blot analysis of human T cell line extract with CD20(ABIN96485) antibody

Publications

Mason, Comans-Bitter, Cordell et al.: "Antibody L26 recognizes an intracellular epitope on the B-cell-associated CD20 antigen." in: The American journal of pathology, Vol. 136, Issue 6, pp. 1215-22, 1990 (PubMed).

Cartun, Coles, Pastuszak: "Utilization of monoclonal antibody L26 in the identification and confirmation of B-cell lymphomas. A sensitive and specific marker applicable to formalin-and B5-fixed, paraffin-embedded tissues." in: The American journal of pathology, Vol. 129, Issue 3, pp. 415-21, 1988 (PubMed).

Ishii, Takami, Yuasa et al.: "Two distinct antigen systems in human B lymphocytes: identification of cell surface and intracellular antigens using monoclonal antibodies." in: Clinical and experimental immunology, Vol. 58, Issue 1, pp. 183-92, 1984 (PubMed).

Roggero, Zucca, Mainetti et al.: "Eradication of Borrelia burgdorferi infection in primary marginal zone B-cell lymphoma of the skin." in: Human pathology, Vol. 31, Issue 2, pp. 263-8, 2000 (PubMed).