Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2) antibody

Antigen: Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2)

Synonyms: C3DR, CD21, SLEB9

Clonality: Monoclonal (IF8)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN96502

Additional Information

Alternative name: CD21

Immunogen: Purified receptor for the C3d fragment of the third complement component CR2, from human tonsil.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: IF8

Specificity: This antibody reacts with CD21 antigen of MW 145 kD, present on the surface of mature B cells. The antigen is present at high density on follicular dendritic cells. CD21 is involved in transmitting growth-promoting signals to the interior of the B cell.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with CD21 antibody

Publications

Publications: Petzer, Schulz, Stauder et al.: "Structural and functional analysis of CR2/EBV receptor by means of monoclonal antibodies and limited tryptic digestion." in: Immunology, Vol. 63, Issue 1, pp. 47-53, 1988 (PubMed).

Nemerow, McNaughton, Cooper: "Binding of monoclonal antibody to the Epstein Barr virus (EBV)/CR2 receptor induces activation and differentiation of human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 5, pp. 3068-73, 1985 (PubMed).

Weis, Tedder, Fearon: "Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 3, pp. 881-5, 1984 (PubMed).