CD25 antibody
| Antigen |
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| Clonality |
Monoclonal (IL2R-1) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN96537 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
293.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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BALB/C mice were injected with recombinant human interleukin- 2 receptor.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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IL2R-1
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Specificity
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This antibody is specific to a 55 kD protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD25", type "Antibodies"
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Hosts
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Rat (46), Mouse (34)
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Reactivities
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Mouse (Murine) (45), Human (25), Rat (Rattus) (6), Cow (Bovine) (3), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (76), Immunofluorescence (IF) (9), Immunoprecipitation (IP) (9), Blocking Antibody (Inhibition) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Functional Studies (Func) (2), Immunohistochemistry (IHC) (2), Western Blotting (WB) (2), Blocking Antibody (Inhibition) (1), ELISA (1)
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Conjugates
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FITC (13), PE (11), Biotin (10), APC (5), PE,Cy7 (4), RPE (4), SPRD (4), Alexa Fluor 488 (1), Alexa Fluor 700 (1), PE-Dyomics 647 (1), Pacific Blue (1), RPE,Cy5 (1)
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Alternatives 
