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EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr36)

Details for Product anti-EIF4EBP1 Antibody No. ABIN965486, Supplier: Log in to see
Antigen
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Alternatives
anti-Human EIF4EBP1 antibody for Western Blotting
Epitope
pThr36
57
30
27
23
21
20
17
15
14
14
14
14
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
443
237
195
11
8
5
2
2
1
1
1
Host
Rabbit
388
95
4
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
10
8
8
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
320
201
199
89
61
28
18
15
14
6
5
4
4
3
3
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
Specificity 4E-BP1 (phospho-Thr36)) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gingras, Raught, Gygi, Niedzwiecka, Miron, Burley, Polakiewicz, Wyslouch-Cieszynska, Aebersold, Sonenberg: "Hierarchical phosphorylation of the translation inhibitor 4E-BP1." in: Genes & development, Vol. 15, Issue 21, pp. 2852-64, 2001 (PubMed).