Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr36) antibody

Details for Product No. ABIN965486
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Antigen
Synonyms
153432_at, 43-BP, 4E-BP, 4E-BP1, 4EBP, 4e-bp, 4eBP, 4ebp, BcDNA.HL08053, BcDNA:HL08053, CG8846, CT8705, Dm 4E-BP, Dmel\\CG8846, Phas1, Thor/EIF4EBP, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, d4e-bp, ... show more
153432_at, 43-BP, 4E-BP, 4E-BP1, 4EBP, 4e-bp, 4eBP, 4ebp, BcDNA.HL08053, BcDNA:HL08053, CG8846, CT8705, Dm 4E-BP, Dmel\\CG8846, Phas1, Thor/EIF4EBP, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, d4e-bp, d4eBP, dthor, eIF4E-BP, l(2)06270, l(2)k07736, l(2)k13517, pp20, thor, EIF4EBP1, fc04h09, wu:fc04h09, zgc:64137, 4EBP1, BP-1, PHAS-I, 4e-bp1, AA959816 show less
Epitope
pThr36
(42), (25), (24), (22), (19), (18), (18), (18), (13), (12), (11), (10), (9), (8), (7), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(367), (240), (189), (60), (60), (37), (24), (1)
Host
Rabbit
(345), (56), (21)
Conjugate
Un-conjugated
(21), (18), (11), (10), (7), (7), (7), (7), (7), (7), (7), (7), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(275), (169), (158), (82), (69), (35), (23), (21), (12), (9), (6), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN965486
398.75 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
Specificity 4E-BP1 (phospho-Thr36)) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name 4E-BP1
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gingras, Raught, Gygi et al.: "Hierarchical phosphorylation of the translation inhibitor 4E-BP1." in: Genes & development, Vol. 15, Issue 21, pp. 2852-64, 2001 (PubMed).

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