You are viewing an incomplete version of our website. Please click to reload the website as full version.

EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr45)

Details for Product anti-EIF4EBP1 Antibody No. ABIN965487, Supplier: Login to see
Antigen
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
Alternatives
anti-Human EIF4EBP1 antibody for Western Blotting
Epitope
pThr45
59
31
29
22
21
21
17
14
14
14
13
13
13
12
11
10
10
8
8
6
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
424
233
185
10
8
5
2
2
1
1
1
Host
Rabbit
389
74
9
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
7
7
6
5
5
5
5
5
5
5
5
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
302
197
173
71
57
26
23
18
14
7
6
5
4
3
3
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
Specificity 4E-BP1 (phospho-Thr45) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Moody, Scott, Amirghahari et al.: "Modulation of the cell growth regulator mTOR by Epstein-Barr virus-encoded LMP2A." in: Journal of virology, Vol. 79, Issue 9, pp. 5499-506, 2005 (PubMed).

Kumar, Pandey, Sabatini et al.: "Functional interaction between RAFT1/FRAP/mTOR and protein kinase cdelta in the regulation of cap-dependent initiation of translation." in: The EMBO journal, Vol. 19, Issue 5, pp. 1087-97, 2000 (PubMed).

Gingras, Kennedy, OLeary et al.: "4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway." in: Genes & development, Vol. 12, Issue 4, pp. 502-13, 1998 (PubMed).

Background publications Brugarolas, Lei, Hurley et al.: "Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex." in: Genes & development, Vol. 18, Issue 23, pp. 2893-904, 2004 (PubMed).

Burnett, Barrow, Cohen et al.: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998 (PubMed).