Actin, gamma 1 antibody (C-Term)

Catalog No. ABIN965509

Product Details anti-Actin, gamma 1 Antibody

Target: Actin, gamma 1 (ACTG1)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Actin, cytoplasmic 2 (ACTG1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Actin , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Preservative: Thimerosal (Merthiolate), Sodium azide

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-Actin, gamma 1 antibody (ABIN965509)

Goebel, Brockmann, Bönnemann, Warlo, Hanefeld, Labeit, Durling, Laing: "Actin-related myopathy without any missense mutation in the ACTA1 gene." in: Journal of child neurology, Vol. 19, Issue 2, pp. 149-53, (2004) (PubMed).

Laing, Clarke, Dye, Liyanage, Walker, Kobayashi, Shimakawa, Hagiwara, Ouvrier, Sparrow, Nishino, North, Nonaka: "Actin mutations are one cause of congenital fibre type disproportion." in: Annals of neurology, Vol. 56, Issue 5, pp. 689-94, (2004) (PubMed).

Gunning, Ponte, Okayama, Engel, Blau, Kedes et al.: "Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently ..." in: Molecular and cellular biology, Vol. 3, Issue 5, pp. 787-95, (1983) (PubMed).

Target Details for Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Alternative Name: Actin, cytoplasmic 2 (ACTG1 Products)

Synonyms: ACT antibody, ACTG antibody, BRWS2 antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, ACTA1 antibody, actg1a antibody, Actg antibody, actg1b antibody, AL023024 antibody, Actl antibody, E51 antibody, actin antibody, cytoplasmic antibody, actin gamma 1 antibody, actin, gamma 1 a antibody, actin, gamma 1 antibody, actin gamma 1 S homeolog antibody, actin, gamma, cytoplasmic 1 antibody, Actin, gamma 1 antibody, ACTG1 antibody, actg1 antibody, Actg1 antibody, actg1.S antibody

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization