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FOXO4 antibody (Forkhead Box O4) (pSer197)

Details for Product anti-FOXO4 Antibody No. ABIN965516, Supplier: Log in to see
Antigen
  • FOXO4
  • foxo4
  • AFX
  • AFX1
  • MLLT7
  • Afxh
  • Mllt7
  • afx
  • RGD1561201
  • forkhead box O4
  • similar to Putative fork head domain transcription factor AFX1 (Forkhead box protein O4)
  • FOXO4
  • foxo4
  • LOC100348179
  • Foxo4
Alternatives
anti-Human FOXO4 antibody for Western Blotting
Epitope
pSer197
39
34
25
21
14
9
7
6
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
252
170
116
13
10
6
6
6
4
3
2
1
1
Host
Rabbit
227
17
8
Conjugate
This FOXO4 antibody is un-conjugated
6
6
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
188
94
76
39
37
33
20
8
5
3
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
Specificity AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197. .
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AFX (FOXO4 Antibody Abstract)
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Di, Hao, Gao, Wang, Wang, Qiu, Wen, Zhou, Wu, Lu, Liao, Mei, Zheng, Che: "Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients." in: Clinical and diagnostic laboratory immunology, Vol. 12, Issue 1, pp. 135-40, 2005 (PubMed).

Kops, de Ruiter, De Vries-Smits, Powell, Bos, Burgering: "Direct control of the Forkhead transcription factor AFX by protein kinase B." in: Nature, Vol. 398, Issue 6728, pp. 630-4, 1999 (PubMed).

Background publications Brownawell, Kops, Macara, Burgering: "Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX." in: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3534-46, 2001 (PubMed).