Forkhead Box O4 (FOXO4) (pSer197) antibody

Details for Product No. ABIN965516
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Antigen
Synonyms FOXO4, foxo4, AFX, AFX1, MLLT7, Afxh, Mllt7, afx, RGD1561201
Epitope
pSer197
(44), (26), (22), (20), (8), (7), (6), (6), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(209), (140), (95), (49), (36), (36), (36), (2), (1)
Host
Rabbit
(203), (5), (2)
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(162), (71), (57), (40), (39), (27), (23), (11), (6), (1), (1)
Pubmed 3 references available
Quantity 100 μg
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Catalog No. ABIN965516
398.75 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
Specificity AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197. .
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AFX
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Kops, de Ruiter, De Vries-Smits et al.: "Direct control of the Forkhead transcription factor AFX by protein kinase B." in: Nature, Vol. 398, Issue 6728, pp. 630-4, 1999 (PubMed).

Brownawell, Kops, Macara et al.: "Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX." in: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3534-46, 2001 (PubMed).

Di, Hao, Gao et al.: "Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients." in: Clinical and diagnostic laboratory immunology, Vol. 12, Issue 1, pp. 135-40, 2005 (PubMed).

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