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Forkhead Box O4 (FOXO4) (pSer197) antibody

Antigen

Forkhead Box O4 (FOXO4)
Synonyms AFX, AFX1, MLLT7, MGC120490, afx, Afxh, Mllt7, MGC117660, RGD1561201, FOXO4, foxo4
Binding Site
Alternatives

pSer197
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965516
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name AFX
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
Specificity AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197. .

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Kops, de Ruiter, De Vries-Smits et al.: "Direct control of the Forkhead transcription factor AFX by protein kinase B." in: Nature, Vol. 398, Issue 6728, pp. 630-4, 1999 (PubMed).

Brownawell, Kops, Macara et al.: "Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX." in: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3534-46, 2001 (PubMed).

Di, Hao, Gao et al.: "Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients." in: Clinical and diagnostic laboratory immunology, Vol. 12, Issue 1, pp. 135-40, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Forkhead Box O4 (FOXO4)", type "Antibodies"
Hosts Rabbit (105), Goat (5), Mouse (2)
Reactivities Human (109), Mouse (Murine) (76), Rat (Rattus) (68), Cow (Bovine) (35), Dog (Canine) (35), Chicken (34), Horse (Equine) (33), Pig (Porcine) (10), Hamster (2), Cat (Feline) (1)
Applications Western Blotting (WB) (77), Immunofluorescence (IF) (44), ELISA (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunohistochemistry (IHC) (19), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer197 (14), pSer193 (7), C-Term (6), pSer262 (6), Internal Region (5), N-Term (5), Ser197 (4), AA 59-73 (1), Phosphospecific (1), pSer256 (1), pThr32 (1), pThr451 (1), phospho (1)