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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Synonyms
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ... show more
Binding Site
Alternatives

pSer473
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965523
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Akt
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).
Specificity Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.
Synonyms AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Baudhuin, Jiang, Zaslavsky et al.: "S1P3-mediated Akt activation and cross-talk with platelet-derived growth factor receptor (PDGFR)." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 2, pp. 341-3, 2004 (PubMed).

Feng, Tamaskovic, Yang et al.: "Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 35510-7, 2004 (PubMed).

Min, Cheong, Kim et al.: "Cytoplasmic mislocalization of p27Kip1 protein is associated with constitutive phosphorylation of Akt or protein kinase B and poor prognosis in acute myelogenous leukemia." in: Cancer research, Vol. 64, Issue 15, pp. 5225-31, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts Rabbit (370), Mouse (95), Sheep (6), Goat (3), Chicken (1)
Reactivities Human (464), Mouse (Murine) (288), Rat (Rattus) (284), Chicken (114), Dog (Canine) (111), Cow (Bovine) (93), Sheep (Ovine) (88), Pig (Porcine) (83), Rabbit (37), Monkey (21), Chimpanzee (5), Horse (Equine) (5), Cat (Feline) (4), Chemical (3), Xenopus laevis (2), Arabidopsis thaliana (1), Frog (1)
Applications Western Blotting (WB) (347), ELISA (231), Immunofluorescence (IF) (151), Immunohistochemistry (IHC) (115), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (84), Flow Cytometry (FACS) (77), Immunoprecipitation (IP) (39), Immunocytochemistry (ICC) (20), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Dot Blot (Dot) (7), Fluorescence Microscopy (FM) (5), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (DB) (1)
Conjugates Biotin (12), Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (7), HRP (5), PE (5), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), APC (1), Agarose Beads (1), Atto 594 (1), CF (1), Sepharose (1)
Epitopes pSer473 (38), pThr308 (28), C-Term (21), pTyr474 (19), pSer473,pTyr474 (18), N-Term (15), Internal Region (14), pThr450 (12), pSer129 (8), Ser473 (7), pThr308,Internal Region (7), pTyr326 (7), Thr308 (6), pSer124 (4), pThr34 (4), Ser124 (3), Ser129 (3), Thr450 (3), pSer246 (3), AA 460-480 (2), Ser246 (2), pSer473,Internal Region (2), AA 2-480 (1), AA 302-311,pThr308 (1), AA 460-480,C-Term, pSer473 (1), AA 464-477 (1), AA 74-89 (1), AA 80 (1), C-Term,Thr450 (1), N-Term,Gly37 (1), Phosphospecific (1), Tyr326 (1), pSer269 (1), pSer308 (1), pThr500 (1), pThr72 (1), pTyr204 (1), pTyr312,pTyr316,pTyr315 (1)