Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr174) antibody

Details for Product No. ABIN965547
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Antigen
Synonyms Prkaa1, ampk, ampka1, PRKAA1, AMPK, AMPKa1, AMPKalpha1, AI194361, AI450832, AL024255, C130083N04Rik, cb116, im:7154392, sb:cb130, wu:fa94c10, AMPK1
Epitope
pThr174
(23), (19), (16), (14), (14), (12), (11), (9), (8), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(200), (118), (111), (30), (28), (28), (28), (4), (3), (1), (1), (1)
Host
Rabbit
(187), (31), (1)
Conjugate
Un-conjugated
(9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Immunohistochemistry (IHC)
(163), (78), (60), (41), (41), (24), (19), (8), (7), (6), (3), (1), (1)
Pubmed 3 references available
Quantity 100 μg
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Catalog No. ABIN965547
385.00 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
Specificity AMPK1 (Phospho-Thr174) antibody detects endogenous levels of AMPK1 only when phosphorylated at threonine 174.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AMPK1
Application Notes IHC: 1:50-1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: da Silva Xavier, Leclerc, Salt et al.: "Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 8, pp. 4023-8, 2000 (PubMed).

Woods, Johnstone, Dickerson et al.: "LKB1 is the upstream kinase in the AMP-activated protein kinase cascade." in: Current biology : CB, Vol. 13, Issue 22, pp. 2004-8, 2003 (PubMed).

Hurley, Anderson, Franzone et al.: "The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29060-6, 2005 (PubMed).

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