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Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr174) antibody

Antigen

Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)
Synonyms
PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C1 ... show more
Binding Site
Alternatives

pThr174
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965547
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name AMPK1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
Specificity AMPK1 (Phospho-Thr174) antibody detects endogenous levels of AMPK1 only when phosphorylated at threonine 174.
Synonyms PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Application Details
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Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product da Silva Xavier, Leclerc, Salt et al.: "Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 8, pp. 4023-8, 2000 (PubMed).

Woods, Johnstone, Dickerson et al.: "LKB1 is the upstream kinase in the AMP-activated protein kinase cascade." in: Current biology : CB, Vol. 13, Issue 22, pp. 2004-8, 2003 (PubMed).

Hurley, Anderson, Franzone et al.: "The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29060-6, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)", type "Antibodies"
Hosts Rabbit (161), Mouse (12)
Reactivities Human (147), Mouse (Murine) (113), Rat (Rattus) (111), Cow (Bovine) (56), Chicken (55), Dog (Canine) (55), Pig (Porcine) (55), Horse (Equine) (54), Sheep (Ovine) (18), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1), Zebrafish (1)
Applications Western Blotting (WB) (98), Immunofluorescence (IF) (75), ELISA (58), Immunohistochemistry (IHC) (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Immunoprecipitation (IP) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Flow Cytometry (FACS) (8), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (4), Dot Blot (DB) (1), Dot Blot (Dot) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Radioimmunoassay (RIA) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes C-Term (26), pThr172 (19), pThr198 (17), pSer485,Ser491 (13), N-Term (8), pSer487 (7), pThr174 (6), pSer491,pSer485 (5), Ser487 (4), pSer485 (4), AA 376-392 (1), C-Term,AA 480-510 (1), C-Term,AA 489-518 (1), Internal Region (1), Ser485, Ser491 (1), Thr174 (1), pSer178 (1), pSer485, pSer491 (1), pSer487,pSer491 (1), pT172 (1)