You are viewing an incomplete version of our website. Please click to reload the website as full version.

AMPK1/AMPK2 (pSer485), (pSer491) antibody

Details for Product No. ABIN965549, Supplier: Log in to see
Antigen
Epitope
pSer485, pSer491
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
6
6
6
Host
Rabbit
6
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
6
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- SP-V-S).
Specificity AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at serine 485 or serine 491.
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Kim, Sekiya, Poulin, Bae, Rhee: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, 2004 (PubMed).

Humphries, Dangelmaier, Sommer, Kipp, Kato, Griffith, Bakman, Turk, Daniel, Rawlings: "Tec kinases mediate sustained calcium influx via site-specific tyrosine phosphorylation of the phospholipase Cgamma Src homology 2-Src homology 3 linker." in: The Journal of biological chemistry, Vol. 279, Issue 36, pp. 37651-61, 2004 (PubMed).

Suzuki-Inoue, Wilde, Andrews, Auger, Siraganian, Sekiya, Rhee, Watson: "Glycoproteins VI and Ib-IX-V stimulate tyrosine phosphorylation of tyrosine kinase Syk and phospholipase Cgamma2 at distinct sites." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 1023-9, 2004 (PubMed).

Rodriguez, Matsuda, Storey, Katan: "Requirements for distinct steps of phospholipase Cgamma2 regulation, membrane-raft-dependent targeting and subsequent enzyme activation in B-cell signalling." in: The Biochemical journal, Vol. 374, Issue Pt 1, pp. 269-80, 2003 (PubMed).