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PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (pSer485)

Details for Product anti-PRKAA2 Antibody No. ABIN965549, Supplier: Login to see
Antigen
  • ampk
  • ampk2
  • prkaa
  • prkaa1
  • PRKAA2
  • AMPK
  • AMPK2
  • AMPKa2
  • PRKAA
  • Ampk
  • Ampka2
  • 2310008I11Rik
  • A830082D05
  • AMPKalpha2
  • AMPKA2
Alternatives
anti-Human PRKAA2 antibody for Western Blotting
Epitope
pSer485, pSer491
46
18
16
16
15
14
13
13
13
6
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
220
83
65
6
4
4
2
1
1
1
1
Host
Rabbit
186
25
17
1
Clonality
Polyclonal
Conjugate
This PRKAA2 antibody is un-conjugated
11
11
11
8
8
8
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
171
103
60
33
23
22
16
13
6
3
2
1
1
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- SP-V-S).
Specificity AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at serine 485 or serine 491.
Alternative Name AMPK1/AMPK2 (PRKAA2 Antibody Abstract)
Pathways AMPK Signaling
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Kim, Sekiya, Poulin et al.: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, 2004 (PubMed).

Humphries, Dangelmaier, Sommer et al.: "Tec kinases mediate sustained calcium influx via site-specific tyrosine phosphorylation of the phospholipase Cgamma Src homology 2-Src homology 3 linker." in: The Journal of biological chemistry, Vol. 279, Issue 36, pp. 37651-61, 2004 (PubMed).

Suzuki-Inoue, Wilde, Andrews et al.: "Glycoproteins VI and Ib-IX-V stimulate tyrosine phosphorylation of tyrosine kinase Syk and phospholipase Cgamma2 at distinct sites." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 1023-9, 2004 (PubMed).

Rodriguez, Matsuda, Storey et al.: "Requirements for distinct steps of phospholipase Cgamma2 regulation, membrane-raft-dependent targeting and subsequent enzyme activation in B-cell signalling." in: The Biochemical journal, Vol. 374, Issue Pt 1, pp. 269-80, 2003 (PubMed).