Apolipoprotein M antibody (APOM)

Details for Product anti-APOM Antibody No. ABIN965579, Supplier: Log in to see
Antigen
  • APOM
  • fb62d12
  • wu:fb62d12
  • g3a
  • ng20
  • hspc336
  • G3a
  • HSPC336
  • NG20
  • apo-M
  • 1190010O19Rik
  • apolipoprotein M
  • apom
  • APOM
  • Apom
  • LOC100350574
Reactivity
Human
128
59
32
6
5
4
2
2
1
1
Host
82
63
29
Clonality
Monoclonal
Conjugate
This Apolipoprotein M antibody is un-conjugated
13
9
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
151
117
45
34
28
13
12
8
5
4
2
2
1
1
1
Options
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Isotype IgG1, IgG2b
Specificity Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3)
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name ApoM (APOM Antibody Abstract)
Background ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21kDa),is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.
Gene ID 55937
Application Notes Western Blot: 1: 500- 1: 2,000
IF: 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Product cited in: Zhang, Jiao, Hurtig, Dong, Zheng, Luo, Nilsson-Ehle, Ye, Xu: "Expression pattern of apolipoprotein M during mouse and human embryogenesis." in: Acta histochemica, Vol. 106, Issue 2, pp. 123-8, 2004 (PubMed).

Duan, Dahlbäck, Villoutreix: "Proposed lipocalin fold for apolipoprotein M based on bioinformatics and site-directed mutagenesis." in: FEBS letters, Vol. 499, Issue 1-2, pp. 127-32, 2001 (PubMed).

Xu, Dahlbäck: "A novel human apolipoprotein (apoM)." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31286-90, 1999 (PubMed).

Background publications Xu, Nilsson-Ehle, Ahrén: "Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects." in: The Journal of nutritional biochemistry, Vol. 15, Issue 10, pp. 579-82, 2004 (PubMed).