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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody

Antigen

Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5)
Synonyms ASK1, MEKK5, MAPKKK5, MAP3K5, Ask1, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11
Binding Site
Alternatives

pSer966
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965599
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name ASK1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).
Specificity ASK1 (phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.

Application Details
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Application Notes IHC: 1:50~1:100
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cardiovascular, Hypertrophy, Apoptosis/Necrosis
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Goldman, Chen, Fu: "Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 10442-9, 2004 (PubMed).

Fujii, Goldman, Park et al.: "Negative control of apoptosis signal-regulating kinase 1 through phosphorylation of Ser-1034." in: Oncogene, Vol. 23, Issue 29, pp. 5099-104, 2004 (PubMed).

Zhang, Zheng, Storz et al.: "Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 19036-44, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5)", type "Antibodies"
Hosts Rabbit (164), Mouse (5)
Reactivities Human (167), Mouse (Murine) (56), Rat (Rattus) (41), Dog (Canine) (15), Horse (Equine) (15), Chicken (10), Cow (Bovine) (5)
Applications Western Blotting (WB) (102), ELISA (69), Immunofluorescence (IF) (59), Immunohistochemistry (IHC) (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunocytochemistry (ICC) (5), Immunoprecipitation (IP) (5), Flow Cytometry (FACS) (4), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer83 (28), pSer966 (20), C-Term (12), N-Term (7), pSer1033 (7), Ser83 (4), Ser966 (4), pSer967 (4), pThr845 (4), Ser1033 (2), Thr842 (2), AA 1198-1216 (1), AA 1276-1291 (1), AA 280 (1), AA 76-87,pSer83 (1), Internal Region (1), pSer192 (1), pSer260 (1), pSer428 (1), pThr668 (1)