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BCL2-Associated Agonist of Cell Death (BAD) (pSer112) antibody

Antigen

BCL2-Associated Agonist of Cell Death (BAD)
Synonyms BBC2, BCL2L8, wu:fa01b12, wu:fa96d04, MGC72439, BAD, MGC127164
Binding Site
Alternatives

pSer112
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965635
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name BAD
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-SP-Y-P).
Specificity BAD (phospho-Ser112) phosphorylated at serine 112.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Apoptosis/Necrosis
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Wang, Sun, Paller: "Inhibition of integrin-linked kinase/protein kinase B/Akt signaling: mechanism for ganglioside-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44504-11, 2001 (PubMed).

Rice, Washington, Schleman et al.: "Sulindac sulfide inhibits epidermal growth factor-induced phosphorylation of extracellular-regulated kinase 1/2 and Bad in human colon cancer cells." in: Cancer research, Vol. 63, Issue 3, pp. 616-20, 2003 (PubMed).

Zhang, Zhang, Shacter: "Rac1 inhibits apoptosis in human lymphoma cells by stimulating Bad phosphorylation on Ser-75." in: Molecular and cellular biology, Vol. 24, Issue 14, pp. 6205-14, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "BCL2-Associated Agonist of Cell Death (BAD)", type "Antibodies"
Hosts Rabbit (198), Mouse (11), Chicken (2), Rat (1)
Reactivities Human (170), Mouse (Murine) (133), Rat (Rattus) (120), Horse (Equine) (10), Pig (Porcine) (10), Cow (Bovine) (7), Dog (Canine) (7), Cat (Feline) (1), Chicken (1), Monkey (1)
Applications Western Blotting (WB) (125), Immunofluorescence (IF) (74), Immunohistochemistry (IHC) (74), ELISA (65), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunoprecipitation (IP) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Flow Cytometry (FACS) (3), Immunohistochemistry (Fixed) (IHC (fx)) (3), Cellular Assay (CA) (2), Immunoassay (IA) (2), Immunoelectron Microscopy (IEM) (2), Immunostaining (ISt) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 488 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), PE,Cy5.5 (10), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer155 (17), pSer112 (16), pSer136 (15), C-Term (7), pSer118 (7), pSer134 (7), pSer75 (7), pSer99 (7), Internal Region (6), N-Term (4), pSer128 (4), Ser112 (3), Ser136 (2), AA 100-130 (1), AA 19-35 (1), AA 2-168 (1), AA 2-23 (1), AA 51-61 (1), AA 62-73 (1), AA 87-103 (1), BH3 Domain (1), Center (1), Cleaved-Asp71 (1), Middle Region (1), Phosphospecific (1), Pro102 (1), pSer112,pSer75 (1), pSer123 (1), pSer180 (1), pSer498 (1), pSer775 (1), pSer91 (1), pTyr860,pTyr859 (1), phospho (1)