BCL (pThr56) antibody

Antigen: BCL

Binding Site: pThr56

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965647

Additional Information

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).

Specificity: BCL-2 (phospho-Thr56) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56.

Application Details

Application Notes: IHC: 1:50-1:100.

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, 1998 (PubMed).

Siegert, Robbins: "Rb inhibits the intrinsic kinase activity of TATA-binding protein-associated factor TAFII250." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 846-54, 1999 (PubMed).

Yamamoto, Ichijo, Korsmeyer: "BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8469-78, 2000 (PubMed).

Deng, Xiao, Lang et al.: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, 2001 (PubMed).

Huang, Cidlowski: "Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 8, pp. 825-32, 2002 (PubMed).