Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) (pSer69,pSer65) antibody

Antigen

BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)
Synonyms BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim
Binding Site
Alternatives

pSer69,pSer65
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN965668
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name BIM
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human BIM around the phosphorylation site of serine 69 (P-A-SP-P-G).
Specificity BIM (phospho-Ser69/65) antibody detects endogenous levels of BIM only when phosphorylated at serine 69/65.

Application Details
Publications Alternatives back to top

Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Luciano, Jacquel, Colosetti et al.: "Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function." in: Oncogene, Vol. 22, Issue 43, pp. 6785-93, 2003 (PubMed).

Fukazawa, Noguchi, Masumi et al.: "BimEL is an important determinant for induction of anoikis sensitivity by mitogen-activated protein/extracellular signal-regulated kinase kinase inhibitors." in: Molecular cancer therapeutics, Vol. 3, Issue 10, pp. 1281-8, 2004 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)", type "Antibodies"
Hosts Rabbit (100), Hamster (7), Mouse (5), Rat (5), Goat (1)
Reactivities Human (102), Mouse (Murine) (66), Rat (Rattus) (54), Cow (Bovine) (28), Dog (Canine) (28), Sheep (Ovine) (28), Horse (Equine) (23), Pig (Porcine) (23), Monkey (1)
Applications Western Blotting (WB) (79), Immunofluorescence (IF) (42), Immunohistochemistry (IHC) (29), ELISA (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunocytochemistry (ICC) (16), Immunoprecipitation (IP) (13), Flow Cytometry (FACS) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes Internal Region (8), N-Term (8), pSer69 (6), pSer55 (5), AA 22-40 (4), pSer87 (4), Center (3), Ser69 (3), pSer65, pSer69 (3), AA 2-198 (2), BH3 Domain (2), pSer65 (2), AA 145-165 (1), AA 20-40 (1), C-Term (1), Middle Region (1), Middle Region 2 (1), Ser65, Ser69 (1), pSer155 (1), pSer218 (1), pSer69,pSer65 (1)