CD28 (CD28) antibody

Antigen: CD28 (CD28)

Synonyms: Tp44, MGC138290

Clonality: Monoclonal (37-58)

Host: Syrian Hamster

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN96567

Additional Information

Alternative name: CD28

Isotype: IgG  (Matching secondary antibodies)

Clone: 37-58

Description: CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-?.

Specificity: Anti-Mouse CD28 recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation. Antigen Distribution: Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells.

Application Details

Application Notes: IHC (Frozen), FC, IP, B, FA Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD28. A suggested method would be to stain cells expressing CD28 with 0.5 g per 1 x 106 cells in a total staining volume of 100 l followed by Goat Anti-Syrian Hamster IgG (H&L)-FITC (Leinco Prod. No.: S229). Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each Investigator should determine their own optimal working dilution for specific applications.

Concentration: 1.0 - 5.0 mg/ml

Purity: >98% by SDS Page and HPLC

Buffer: Low Endotoxin Functional Formulation

Storage: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at 80°C. Avoid Repeated Freeze Thaw Cycles.

Research Area: CD Antigens

Restrictions: For Research Use only