Breast Cancer 1, Early Onset (BRCA1) (pSer1524) antibody

Antigen: Breast Cancer 1, Early Onset (BRCA1)

Synonyms: BRCA1, iris, pscp, brcai, brcc1, rnf53, brca1, LOC100224649, IRIS, PSCP, BRCAI, BRCC1, RNF53, BROVCA1, ARABIDOPSIS THALIANA BREAST CANCER SUSCEPTIBILITY1, ATBRCA1, breast cancer susceptibility1

Binding Site: pSer1524

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965695

Additional Information

Alternative name: BRCA1

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E).

Specificity: BRCA1 (phospho-Ser1524) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1524.

Application Details

Application Notes: IHC: 1:50-1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Research Area: Cancer, Transcription Factors, Proteolysis / Ubiquitin, DNA/RNA

Restrictions: For Research Use only

Publications

Product: Beger, Pierce, Kruger et al.: "Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 1, pp. 130-5, 2001 (PubMed).

Gardner, Liu: "BRCA1 function in T lymphocytes: a cellular specificity of a different kind." in: Breast cancer research : BCR, Vol. 3, Issue 1, pp. 11-3, 2001 (PubMed).

Zheng, Annab, Afshari et al.: "BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 17, pp. 9587-92, 2001 (PubMed).

Zhang, Willers, Feng et al.: "Chk2 phosphorylation of BRCA1 regulates DNA double-strand break repair." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 708-18, 2003 (PubMed).

Lin, Li, Stewart et al.: "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 17, pp. 6484-9, 2004 (PubMed).