C-ErbB-2 Oncoprotein antibody

Antigen: C-ErbB-2 Oncoprotein

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN965710

Additional Information

Immunogen: Synthetic human c-erbB-2 oncoprotein from intracytoplasmatic part of the c-erbB-2 oncoprotein.

Description: Purified immunoglobulin fraction of rabbit antiserum prediluted in Primary Antibody diluting Buffer pH 7.6.

Specificity: This antibody is specific against human c-erbB-2 oncoprotein.

Comments:

Recommended Positive Control: Breast AdenoCarcinomas, Ovary, Gastrointestinal Tract

Application Details

Application Notes: This antibody is prediluted for Immunohistochemistry use.
Staining Procedure: The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffinembedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with an immunoperoxidase staining kit. Prolonged fixation in buffered formalin can destroy the epitope.

Concentration: 0.007 mg/ml.

Purification: Prediluted.

Storage: Refrigerate at 4°C. Do not freeze.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Product: Soomro, Shousha, Taylor et al.: "c-erbB-2 expression in different histological types of invasive breast carcinoma." in: Journal of clinical pathology, Vol. 44, Issue 3, pp. 211-4, 1991 (PubMed).

May, Mouriesse, May-Levin et al.: "Human breast cancer: identification of populations with a high risk of early relapse in relation to both oestrogen receptor status and c-erbB-2 overexpression." in: British journal of cancer, Vol. 62, Issue 3, pp. 430-5, 1990 (PubMed).

Barnes: "Breast cancer and a proto-oncogene." in: BMJ (Clinical research ed.), Vol. 299, Issue 6707, pp. 1061-2, 1990 (PubMed).

Wright, Angus, Nicholson et al.: "Expression of c-erbB-2 oncoprotein: a prognostic indicator in human breast cancer." in: Cancer research, Vol. 49, Issue 8, pp. 2087-90, 1989 (PubMed).

Bouchard, Lamarre, Tremblay et al.: "Stochastic appearance of mammary tumors in transgenic mice carrying the MMTV/c-neu oncogene." in: Cell, Vol. 57, Issue 6, pp. 931-6, 1989 (PubMed).

Varley, Swallow, Brammar et al.: "Alterations to either c-erbB-2(neu) or c-myc proto-oncogenes in breast carcinomas correlate with poor short-term prognosis." in: Oncogene, Vol. 1, Issue 4, pp. 423-30, 1988 (PubMed).

Slamon, Clark, Wong et al.: "Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene." in: Science (New York, N.Y.), Vol. 235, Issue 4785, pp. 177-82, 1987 (PubMed).