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Jun Proto-Oncogene (JUN) (pSer63) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Binding Site
Alternatives

pSer63
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN965715
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name c-Jun
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D).
Specificity C-Jun (phospho-Ser63) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 63.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Immunology, Innate Immunity, Transcription Factors
Restrictions For Research Use only

Publications
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Product Hurd, Waldron, Rozengurt: "Protein kinase D complexes with C-Jun N-terminal kinase via activation loop phosphorylation and phosphorylates the C-Jun N-terminus." in: Oncogene, Vol. 21, Issue 14, pp. 2154-60, 2002 (PubMed).

Li, Feng, Porter: "JNK-dependent phosphorylation of c-Jun on serine 63 mediates nitric oxide-induced apoptosis of neuroblastoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4058-65, 2004 (PubMed).

Sevilla, Santos, Barcia et al.: "c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK)." in: Oncogene, Vol. 23, Issue 55, pp. 8950-8, 2004 (PubMed).

Wei, Jin, Schlisio et al.: "The v-Jun point mutation allows c-Jun to escape GSK3-dependent recognition and destruction by the Fbw7 ubiquitin ligase." in: Cancer cell, Vol. 8, Issue 1, pp. 25-33, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (348), Mouse (9), Chicken (1), Goat (1), Sheep (1)
Reactivities Human (350), Rat (Rattus) (198), Mouse (Murine) (189), Cow (Bovine) (87), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (254), Immunohistochemistry (IHC) (124), ELISA (118), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (42), Dot Blot (Dot) (6), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (26), pSer63 (19), pThr239 (19), pTyr170 (18), pThr91 (17), pThr93,pThr91 (17), pSer243 (16), Internal Region (12), pThr93 (12), C-Term (8), pThr249 (8), N-Term (7), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), pThr243 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), Sumoylation Site (1), non-pSer73 (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)