Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8)

Synonyms: CD30, Ki-1, D1S166E, Ki, Cd30, RP23-306F22.1, TNFRSF8, TNFSF8

Clonality: Monoclonal (Ber-H2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96578

Additional Information

Alternative name: CD30

Immunogen: BALB/C mice were injected with L428 cell line cells.

Format: Supernatant (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: Ber-H2

Specificity: This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 10.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Pallesen: "The diagnostic significance of the CD30 (Ki-1) antigen." in: Histopathology, Vol. 16, Issue 4, pp. 409-13, 1990 (PubMed).

Schwarting, Gerdes, Duerkop et al.: "BER-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope." in: Blood, Vol. 74, Issue 5, pp. 1678-89, 1989 (PubMed).

Pallesen, Hamilton-Dutoit: "Ki-1 (CD30) antigen is regularly expressed by tumor cells of embryonal carcinoma." in: The American journal of pathology, Vol. 133, Issue 3, pp. 446-50, 1989 (PubMed).