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CD34 antibody (CD34 Molecule)

Details for Product anti-CD34 Antibody No. ABIN965787, Supplier: Log in to see
Antigen
  • AU040960
Reactivity
Human
739
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122
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38
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29
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10
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2
Host
616
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7
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3
Clonality
Monoclonal
Conjugate
This CD34 antibody is un-conjugated
91
89
54
25
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2
1
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Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
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37
14
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8
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Supplier
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Isotype IgG
Specificity Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3).
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name CD34 (CD34 Antibody Abstract)
Background CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Gene ID 947
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Application Notes Dilution Western Blot: 1: 200- 1: 1,000
IF: 1:25- 1: 100
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Product cited in: Sho, Sho, Nanjo, Kawamura, Masuda, Dalman: "Hemodynamic regulation of CD34+ cell localization and differentiation in experimental aneurysms." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 10, pp. 1916-21, 2004 (PubMed).

Krause, Ito, Fackler, Smith, Collector, Sharkis, May: "Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells." in: Blood, Vol. 84, Issue 3, pp. 691-701, 1994 (PubMed).

Satterthwaite, Burn, Le Beau, Tenen: "Structure of the gene encoding CD34, a human hematopoietic stem cell antigen." in: Genomics, Vol. 12, Issue 4, pp. 788-94, 1992 (PubMed).