CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN965795, Supplier: Log in to see
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternatives
anti-Human CD4 antibody for Activation
Reactivity
Human
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619
234
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36
22
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1
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1
Host
Mouse
1323
573
273
10
4
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
258
222
166
100
48
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40
36
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23
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Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
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431
332
309
289
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142
87
61
39
38
30
30
23
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Options
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Immunogen Human leucocytes
Clone B486 (EDU-2)
Isotype IgG2a
Specificity This antibody reacts with a 55kD single chain transmembrane glycoprotien, CD4, expressed on T helper lymphocytes, dendritic cells, microglial cells, and cortical thymus cells. Characteristic of CD4 is its four extracellular immunoglobulin-like domains which help it bind to HLA class II molecules when CD4+ T cells interact with antigenpresenting cells or target cells. Another interesting aspect of the CD4 receptor is the fact that it also serves as the primary receptor for the HIV virus. This antibody was clustered at the 2nd International Leucocyte Typing Workshop.
Purification Concentrated.
Alternative Name CD4 (CD4 Antibody Abstract)
Gene ID 920
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Application Notes Immunohistochemistry: 1:50-1:100.(Frozen sections only)
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen sections only. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications McDougal, Kennedy, Sligh, Cort, Mawle, Nicholson: "Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule." in: Science (New York, N.Y.), Vol. 231, Issue 4736, pp. 382-5, 1986 (PubMed).

Dalgleish, Beverley, Clapham, Crawford, Greaves, Weiss: "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus." in: Nature, Vol. 312, Issue 5996, pp. 763-7, 1985 (PubMed).

Biddison, Rao, Talle, Goldstein, Shaw: "Possible involvement of the T4 molecule in T cell recognition of class II HLA antigens: evidence from studies of proliferative responses to SB antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 152-7, 1983 (PubMed).

Reinherz, Kung, Goldstein, Schlossman: "Separation of functional subsets of human T cells by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 8, pp. 4061-5, 1979 (PubMed).

Product cited in: Zosimova: "[Gas chromatographic detection of benzene, toluene and xylene in biological matter]." in: Sudebno-meditsinskaia ekspertiza, Vol. 29, Issue 3, pp. 37-8, 1986 (PubMed).