CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (B486 (EDU-2))

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN965795

Additional Information

Alternative name: CD4

Gene ID: 920

Immunogen: Human leucocytes

Isotype: IgG2a

Clone: B486 (EDU-2)

Specificity: This antibody reacts with a 55kD single chain transmembrane glycoprotien, CD4, expressed on T helper lymphocytes, dendritic cells, microglial cells, and cortical thymus cells. Characteristic of CD4 is its four extracellular immunoglobulin-like domains which help it bind to HLA class II molecules when CD4+ T cells interact with antigenpresenting cells or target cells. Another interesting aspect of the CD4 receptor is the fact that it also serves as the primary receptor for the HIV virus. This antibody was clustered at the 2nd International Leucocyte Typing Workshop.

Comments:

Recommended Positive Control: Tonsil, Thymus, Spleen

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.(Frozen sections only)
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen sections only. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Concentration: 0.3 mg/ml.

Purification: Concentrated.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Reinherz, Kung, Goldstein et al.: "Separation of functional subsets of human T cells by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 8, pp. 4061-5, 1979 (PubMed).

McDougal, Kennedy, Sligh et al.: "Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule." in: Science (New York, N.Y.), Vol. 231, Issue 4736, pp. 382-5, 1986 (PubMed).

Zosimova: "[Gas chromatographic detection of benzene, toluene and xylene in biological matter]." in: Sudebno-meditsinskaia ekspertiza, Vol. 29, Issue 3, pp. 37-8, 1986 (PubMed).

Dalgleish, Beverley, Clapham et al.: "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus." in: Nature, Vol. 312, Issue 5996, pp. 763-7, 1985 (PubMed).

Biddison, Rao, Talle et al.: "Possible involvement of the T4 molecule in T cell recognition of class II HLA antigens: evidence from studies of proliferative responses to SB antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 152-7, 1983 (PubMed).